Abstract:
In this study, we have examined the nudibranch specie, Chromodoris kuiteri (Cheney et. al., 2016), using mass spectrometry imaging (MSI). The samples were prepared using regular cryostat sectioning to produce 10 - 20 micron sections for macroscopic spatial distribution, and ultrathin cryosectioning following high-pressure freezing to produce 200 nm sections to allow for further investigation of the microscopic sub-cellular features. During this study we utilised high accuracy orthogonal TOF, with and without post ionisation (Soltwisch et. al, 2020), and axial MALDI TOF instruments to show that latrunculin-A was being specifically stored by Chromodoris kuiteri in the mantle dermal formations in outer rim of the organism. This combination of approaches has the potential to contribute towards more routine methodologies for sub-cellular MSI.
References:
Cheney, K.L., White, A., Mudianta, I.W., Winters, A.E., Quezada, M., Capon, R.J., Mollo, E., Garson, M.J. Choose Your Weaponry: Selective Storage of a Single Toxic Compound, Latrunculin A, by Closely Related Nudibranch Molluscs. Plos One. 2016, 11(1): e0145134
Soltwisch, J., Heijs, B., Koch, A., Vens-Cappell, S., Hoehndorf, J., Dreisewerd, K. MALDI-2 on a Trapped Ion Mobility Quadrupole Time-of-Flight Instrument for Rapid Mass Spectrometry Imaging and Ion Mobility Separation of Complex Lipid Profiles. Anal Chem. 2020, 92, 8697-8703